NEXT GENERATION SEQUENCING SERVICES

 

 

NGS service at Huge-F is equipped with several second-generation sequencing platforms to facilitate biological and medical research in the areas of genomics (DNA-seq), transcriptomics (RNA-seq), epigenomics (methylation-seq) and metagenomics (16S rRNA-seq/shotgun).

 

DNA-seq

Next-generation DNA sequencing makes it possible to rapidly compare the genetic content among samples and identify germline and somatic variants of interest, such as single nucleotide polymorphisms (SNPs), insertions and deletions (indels), copy number variants (CNVs), and other structural variations. Huge-F supports a broad range of applications:

   -- Exome-sequencing

 

                               Nimblegen MedExome

 

   -- Custom targeted DNA sequencing

   -- Whole genome sequencing

 

 

Huge-F supports (targeted) DNA sequencing projects by providing project consultancy to guide you in study design, sample preparation and quality control. We apply standard QC and Quality Assessment of sequence data, and offer optional more advanced data analysis services, such as mapping reads and variant calling.

 

 

RNA-seq

Transcriptome sequencing, also referred to as RNA-seq, provides a precise measurement of transcript levels, but it can also be used for discovering novel transcripts, alternative splicing or allele-specific expression.

 

Huge-F supports transcriptomic studies by providing help in designing your study and guide preparation of samples and quality control, to study RNA expression on a transcriptome-wide or smaller scale. It can include:

 

   -- mRNA, non-coding RNAs

   -- small RNAs (including micro RNAs)

   -- directional RNA sequencing

 

We encourage researchers to contact us at an early stage to discuss the details of their project.

 

We apply standard QC and Quality Assessment of sequence data, and offer optional more advanced data analysis services, such as mapping reads, transcriptome assembly or transcript quantification.

 

 

16S rRNA-seq

Next Generation Sequencing techniques have allowed detailed profiling of bacterial communities in high-throughput and cost effective manners. Sequence variation in the 16S ribosomal RNA gene is well suited to analyze bacterial taxonomic diversity in the gut, throat, ear, skin or any other microbial community.

 

We apply standard QC and Quality Assessment of sequence data, and offer optional more advanced data analysis services such as aligning reads and phylogenetic profiling and annotation.

 

 

Please contact us for further information.

 

 

 

 

 

 

 

 

 

 

 

 

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