EPIGENETIC PROFILING

 

Epigenetics is an emerging frontier of science that involves the study of changes in the regulation of gene activity and expression that are not dependent on gene sequence.

Huge-F provides services to measure epigenetic features, such as DNA-methylation and non-coding RNAs using state of the art genotyping and sequencing platforms.

Huge-F supports epigenetic studies by providing help in designing your study and guide preparation of samples and quality control, to study DNA methylation on a genome-wide or smaller scale. We encourage researchers to contact us at an early stage to discuss the details of their project

 

 

We offer the following assays for methylation analysis:

 

 

The Infinium HumanMethylation450 BeadChip by Illumina (or commonly known as Infinium 450k array) is a cost-effective solution for epigenome-wide association studies (EWAS). The array gives single-base methylation information for over 485.000 CpGs throughout the human genome. It offers a combination of comprehensive, expert-selected coverage, high sample throughput, and affordable price.

 

The Infinium 450K array covers 99% of RefSeq genes, with an average of 17 CpG sites per gene region distributed across the promoter, 5′UTR, first exon, gene body, and 3′UTR. It covers 96% of CpG islands, with additional coverage in island shores and the regions flanking them.

 

Highlights of this assay:

 -- Screen >485.000 known methylation sites

 -- Single base resolution

 -- Low DNA input (500 ng genomic DNA)

 -- Short throughput time

 -- Most cost-effective genome-wide DNA methylation technique

 

 

The Agena Bioscience's MassaARRAY® EpiTYPER assay® is a targeted technique to measure methylation of a focused number of CpGs. The assay is a bisulfite conversion based method and uses base-specific cleavage and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) to measure methylation of specific CpG sites quantitative. It is a flexible technique and is capable to cover CpGs over a region up to 400 bp per assay.

 

Highlights of this assay:

 -- Flexible assay design. Capable to design assay for region of interest

 -- CpG site coverage up to 400 bp regions in one assay

 -- single base resolution

 -- Low DNA input

 

 

 

 

The Illumina methylation-EPIC array has a comprehensive genome-wide coverage of the methylome, featuring:

 -- >95% of CpG islands

 -- sites outside of CpG islands

 -- Non-CpG methylated sites identified in human stem cells

 -- Differentially methylated sites identified in tumor versus normal • FANTOM5 enhancers

 -- ENCODE open chromatin and enhancers

 -- DNase hypersensitive sites

 -- miRNA promoter regions

 -- Suitable for a variety of human tissues

 

 

We offer data-analysis services for analyzing epigenetic data.

 

 

Please contact us for further information.

 

 

 

 

--- on offer today € 275 ---