Epigenetics is an emerging frontier of science that involves the study of changes in the regulation of gene activity and expression that are not dependent on gene sequence.
Huge-F provides services to measure epigenetic features, such as DNA-methylation and non-coding RNAs using state of the art genotyping and sequencing platforms.
Huge-F supports epigenetic studies by providing help in designing your study and guide preparation of samples and quality control, to study DNA methylation on a genome-wide or smaller scale. We encourage researchers to contact us at an early stage to discuss the details of their project
We offer the following assays for methylation analysis:
The Infinium HumanMethylation450 BeadChip by Illumina (or commonly known as Infinium 450k array) is a cost-effective solution for epigenome-wide association studies (EWAS). The array gives single-base methylation information for over 485.000 CpGs throughout the human genome. It offers a combination of comprehensive, expert-selected coverage, high sample throughput, and affordable price.
The Infinium 450K array covers 99% of RefSeq genes, with an average of 17 CpG sites per gene region distributed across the promoter, 5′UTR, first exon, gene body, and 3′UTR. It covers 96% of CpG islands, with additional coverage in island shores and the regions flanking them.
Highlights of this assay:
-- Screen >485.000 known methylation sites
-- Single base resolution
-- Low DNA input (500 ng genomic DNA)
-- Short throughput time
-- Most cost-effective genome-wide DNA methylation technique
The Agena Bioscience's MassaARRAY® EpiTYPER assay® is a targeted technique to measure methylation of a focused number of CpGs. The assay is a bisulfite conversion based method and uses base-specific cleavage and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) to measure methylation of specific CpG sites quantitative. It is a flexible technique and is capable to cover CpGs over a region up to 400 bp per assay.
Highlights of this assay:
-- Flexible assay design. Capable to design assay for region of interest
-- CpG site coverage up to 400 bp regions in one assay
-- single base resolution
-- Low DNA input
The Illumina methylation-EPIC array has a comprehensive genome-wide coverage of the methylome, featuring:
-- >95% of CpG islands
-- sites outside of CpG islands
-- Non-CpG methylated sites identified in human stem cells
-- Differentially methylated sites identified in tumor versus normal • FANTOM5 enhancers
-- ENCODE open chromatin and enhancers
-- DNase hypersensitive sites
-- miRNA promoter regions
-- Suitable for a variety of human tissues
We offer data-analysis services for analyzing epigenetic data.